In-Vitro Antioxidant Potential of Terminalia arjuna Bark Aqueous Extract on Sheep Red Blood Cells
DOI:
https://doi.org/10.53555/ans.v2i5.95Keywords:
Terminalia Arjuna (TA), Osmofragility test,, Lipid peroxidationAbstract
An aqueous extract from the bark of Terminalia arjuna (TA), Marutham Pattai was evaluated for
its protective (antioxidant) effect against hydrogen peroxide (H2O2) induced oxidation in normal
sheep erythrocytes (red blood cells (RBCs). RBCs, treated with increasing concentrations of 30%
H2O2 along with Terminalia arjuna aqueous bark extract (TA) were analyzed for hemolysis and
lipid peroxidation. Sheep RBCs treated with increasing concentrations of glucose, to study effect
of high glucose level or hyperglycemia on normal SRBC, were found to be more susceptible to
lipid peroxidation than those from normal subjects. However, on treatment with TA, the
oxidative modifications in both the groups (RBCs treated with glucose and not treated with
glucose) were found to reduce significantly. The inhibition of lipid peroxidation was
concentration dependent up to 100 μl of extract, which contained 0.75 mM gallic acid equivalent
(GAE) of phenolic compounds. The total phenolic content in the extract was determined
spectrophotometrically according to the Folin-Ciocalteau procedure and was expressed as mg or
mM GAE. The results indicate that the aqueous bark extract of Terminalia arjuna (TA) contains
antioxidants and protects cellular structures from oxidative damage. These findings demonstrate
the potent antioxidant properties of the aqueous bark extract of Terminalia arjuna.
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