The protocol of Agrobacterium tumefaciens-mediated stable transform ation of the 84K Poplar

  • Wang Lina Northeast Forestry University
Keywords: Agrobacterium Transformation, Plant tissue culture, Explant, Genetic transformation


A protocol for successful callus induction and plant regeneration from 84K Poplar is described.The stable transformation protocol will promote the functional characteriz ation of genes in woody plants.


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Author Biography

Wang Lina, Northeast Forestry University

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road,
Harbin ,150040, China


[1] Callus induction and haploid plant regeneration from baby primrose (Pri
mula forbesii Franch.) anther culture
[2]Jin J Z, Liang S, Hui C, et al. An efficient Agrobacterium-mediated transformation
method for the edible mushroom Hypsizygus marmoreus[J]. Microbiological Researc
h, 2014, 169(9-10): 741-748.
[3] Ikeuchi M , Sugimoto K , Iwase A . Plant Callus: Mechanisms of Induction and
Repression[J]. The Plant Cell, 2013, 25(9):3159-3173.
[4] Jia Y , Zhang Q X , Pan H T , et al. Callus induction and haploid plant regener
ation from baby primrose (Primula forbesii Franch.) anther culture[J]. Scientia Horticul
turae, 2014, 176:273-281.
[5]Kayani W K, Fattahi M, Palazòn J, et al. Comprehensive screening of influential f
actors in the Agrobacterium tumefaciens- mediated transformation of the Himalayan
elixir: Ajuga bracteosa, Wall. ex. Benth[J]. Journal of Applied Research on Medicin
al & Aromatic Plants, 2016.
[6]Kalbande B B, Patil A S. Plant tissue culture independent Agrobacterium tumefacie
ns mediated In-planta, transformation strategy for upland cotton (Gossypium hirsutu
m )[J]. Genetic
[7] Li S , Ridenour J B , Kim H , et al. Agrobacterium tumefaciens-mediated transfo
rmation of the soybean pathogen Phomopsis longicolla[J]. Journal of Microbiological
Methods, 2013, 92(3):244-245.
[8] M. Liesebach, G. Naujoks. Approaches on vegetative propagation of difficult-to-ro
ot Salix caprea[J]. Plant Cell Tissue and Organ Culture, 2004, 79: 239-247.
[9] Osman N I , Sidik N J , Awal A . Effects of variations in culture media and ho
rmonal treatments upon callus induction potential in endosperm explant of Barringtonia
racemosa L.[J]. Asian Pacific Journal of Tropical Biomedicine, 2016, 6(2):143-147.
[10] Martins P K, Ribeiro A P, Kobayashi A K, et al. A simple and highly efficient
Agrobacterium-mediated transformation protocol for Setaria viridis[J]. Biotechnology
Reports, 2015, 6: 41-44.
[11] Mallesham Bulle. Agrobacterium tumefaciens-Mediated transformation of Woodfor
dia fruticosa[J]. Journal of Genetic Engineering and Biotechnology. 2015, 13(2): 201
[12] Parons T J, Sinkar V P, Stettler R F, et al. Transformation of Poplar by Agroba
cterium tumefaciens[J]. Biotechnology, 1986, 4(6): 533-536.
[13] Rodrigues M B C, Fávaro L C D L, Pallu A P D S, et al. Agrobacterium -med
iated transformation of Guignardia citricarpa: An efficient tool to gene transfer and
random mutagenesis[J]. Fungal Biology, 2013, 117(8): 556-68.
[14] Shi Q , Liu P , Wang J , et al. A novel in vivo shoot regeneration system via
callus in woody fruit tree Chinese jujube (Ziziphus jujuba Mill.)[J]. Scientia Horticultu
rae, 2015, 188:30-35.
[15] Wu J , Philip A. O’Brien. Stable transformation ofRhizoctonia solaniwith a modi
fied hygromycin resistance gene[J]. Australasian Plant Pathology, 2009, 38(1):79-84.
[16] Ziemienowicz A. Agrobacterium -mediated plant transformation: Factors, applicati
ons and recent advances[J]. Biocatalysis & Agricultural Biotechnology, 2014, 3(4): 9
How to Cite
Lina, W. (2018). The protocol of Agrobacterium tumefaciens-mediated stable transform ation of the 84K Poplar. International Journal For Research In Applied And Natural Science (ISSN: 2208-2085), 4(8), 30-36. Retrieved from