Defatted Seed Meal of Soybean: An Inexpensive Substrate for Alkaline Protease Production from Selected Isolate
Keywords:
alkaline protease,, Cyclobacterium sp, defatted seed meal of soybean,, protease activityAbstract
Industrially important efficient alkaline protease producer was isolated from soil samples collected from mango garden of S.R.T.M. University campus in Nanded district of Maharashtra and identified based on its morphological, microscopic and physiological characters, biochemical pattern and enzyme profile as Cyclobacterium sp. APM2. Production of alkaline protease was carried out in nutrient salt solution supplemented with 10 g/L defatted seed meal of soybean and 350 U/mL production of alkaline protease was recorded after 48 h. APM2 alkaline protease has shown remarkable catalytic efficiency at pH range 7-9 and temperature range 30-60 oC as well. Therefore alkaline protease from Cyclobacterium sp. APM2 can be used in different biotechnological industries where hydrolysis of protein is carried out at alkaline pH and elevated range of temperature.
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