Isolation and Identification of Industrially Important Salt Stable Amylase Producer
DOI:
https://doi.org/10.53555/bp.v2i3.141Keywords:
salt stable amylase,, Halobacillus sp, amylase activity,, starch agarAbstract
Efficient salt stable amylase producer was isolated by using starch agar medium containing 10 % NaCl (pH 7.2) from soil samples collected from over irrigated and water logged sites of Kaleshwar farming area in Nanded district of Maharashtra. The salt stable amylase producer was identified based on its morphological, microscopic and physiological characters, biochemical pattern and enzyme profile as Halobacillus sp. SSA1. Production of salt stable amylase was carried out in starch yeast extract medium containing 10 % NaCl. Production of salt stable amylase was recorded 2103.47 U/mL in cell free supernatant. The crude salt stable amylase has shown maximum catalytic efficiency at pH 7 and 4 oC temperature.
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