Production of Amylase by Penicillium sp. Using Solid State Fermentation Method and Inexpensive Agricultural Residues

Authors

  • Anupama P. Pathak School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra
  • Sachin B. Devkatte School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra
  • Mukundraj G. Rathod School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra

DOI:

https://doi.org/10.53555/bp.v2i4.135

Keywords:

acid amylase,, Penicillium sp, soybean husk, wheat husk,, amylase activity

Abstract

Industrially important amylase producer was isolated from soil samples of S.R.T.M. University campus in Nanded district of Maharashtra and identified based on its morphological and microscopic characters as Penicillium sp. AFS3. Production of amylase was carried out by using wheat and soybean husks in solid state fermentation. 81.80 U/mL production of amylase was recorded after 96 h. AFS3 amylase has shown remarkable catalytic efficiency at pH range 4-6 and temperature range 30-50 oC as well. Therefore amylase from Penicillium sp. AFS3 can be used in different biotechnological industries
where hydrolysis of starch is carried out at low pH and elevated range of temperature.

Downloads

Download data is not yet available.

References

Bhunia, B., Basak, B., Bhattacharya, P., & Dey, A. (2013). Process engineering studies to investigate the effect of temperature and pH on kinetic parameters of alkaline protease production.Journal of bioscience and bioengineering,115(1), 86-89.

Dastager, S. G., Mawlankar, R., Mual, P., Verma, A., Krishnamurthi, S., Joseph, N., & Shouche, Y. S. (2015). Bacillus encimensissp. nov. isolated from marine sediment.International journal of systematic and evolutionary microbiology,65(5), 1421-1425.

Domsch, H.K., Anderson, T.H. and Gams, W. 1980. Compendium of Soil Fungi. Volume I. Academic Press, A subsidiary of Harcourt Brace Jovanovich, Publishers, London.

Gavali, J., & Pathak, A. (2015). Isolation and Identification Of Cold ActiveAmylase Producer From Gastro Intestinal Tract Of Channa Striata. Current Trends in Aquaculture,53.Gilman, J.C. 1957. A Manual of Soil Fungi. (Second Indian Reprint), Oxford and IBH Publishing Co. New Delhi. India.

Ghorbel, R. E., Maktouf, S., Massoud, E. B., Bejar, S., & Chaabouni, S. E. (2009). New thermostable amylase from Bacillus cohniiUS147 with a broad pH applicability.Applied biochemistry and biotechnology,157(1), 50-60.

Khairnar, R. S., Mahabole, M. P., &Pathak, A. P. (2012). Nanoactivator mediated modifications in thermostable amylase from Bacillus licheniformis. Indian J Biochem. Biophy.49, 468-471.

Kolekar, Y.M., Pawar, S. P., Adav,S. S., Zheng,L.,Li,W., Shouche,Y. S., Dastager, S. G., Kodam, K. M. (2013). Alishewanella solinquinatisp. nov., isolated from soil contaminated with textile dyes.Current microbiology,67(4),454-459.

Pathak A. P., Parware S. U., Rathod M. G. (2016). Isolation and identification of industrially important salt stable amylase producer. International Journal for Research in Biology and Pharmacy Research(in press).

Pathak A. P., Lohagave A. G., Rathod M. G. (2015a). Exploration of paper industry effluent for isolation of efficient starchy material degrader to promote bioremediation. Int. J. Adv. Pharm. Biol. Chem. 4(4) 729-736.

Pathak A.P., Jethaliya C.S., Sarsar M.S., Jadhav S.R. (2015b) Isolation and characterisation of potential amylase producing strain from the agriculture waste. Int. J. Adv. Pharm. Biol. Chem,4(4): 829-832.

Pathak, A.P., Lodge, N., Gavali, J.T., & Rathod, M.G. (2015c). Isolation and characterization of cold-active protease producer from ice factory samples. Int. J. Adv. Pharm. Biol. Chem,4, 751-754.

Pathak, A. P., & Rathod, M. G. (2013). Production and characterization of alkaline protease by Bacillus pasteurii: a Lonar Soda Lake Isolate. Innovative Research in Chemistry,1(1), 22-26.

Pathak, A. P., & Rathod, M. G. (2014). Exploration of Unkeshwar hot springs in Maharashtra for thermostable amylase producer.Res. Rev. Biosci,8(7), 269-276.

Pathak, A. P., & Rathod, M. G. (2015). Cultivable bacterial diversity of terrestrial thermal spring of Unkeshwar, India.Journal of Biochemical Technology,5(4), 814-818.

Pathak, A. P., & Sardar, A. G. (2012). Isolation and characterization of carotenoid producing Haloarchaea from solar saltern of Mulund, Mumbai, India.Indian J. Natural Products Resources,3, 483-488.

Pathak, A. P., & Sardar, A. G. (2014). Isolation and characterization of salt stable protease producing archaea from marine solar saltern of Mulund, Mumbai.Indian J. Mar. Sci,43(3), 412-417.

Pathak, A. P., Rathod, M. G., &Rampurkar, V. V. (2014a). An eco-friendly approach for thermostable amylase production using Bacillus firmusAPP6: a hot spring isolate, Asiatic J.Biotech. Res,4(4), 101-105.

Pathak, A. P., Sardar, A. G., & Janaj, P. C. (2014b). Exploring the salted fish for alkaline protease producing bacteria.Indian J. Mar. Sci.,43, 10 (in press).

Pathak, A.P. & Rathod, M.G. (2016) Assessment of diverse thermostable alkaline lipase producers from Unkeshwar hot spring of Maharashtra, India. Concept. Pure Appl. Sci.3(1), 1-9.

Polkade, A. V., Ramana, V. V., Joshi, A., Pardesi, L., & Shouche, Y. S. (2015). Rufibacter immobilis sp. nov., isolated from a high-altitude saline lake.International journal of systematic and evolutionary microbiology,65(5), 1592-1597.

Rathod, M. G., & Pathak, A. P. (2014a). Isolation and identification of alkaline protease producer from selected alkaline habitat.International Journal of Innovative Biological Research,3(1), 1-6.

Rathod, M. G., &Pathak, A. P. (2014b). Wealth from waste: Optimized alkaline protease production from agro-industrial residues by Bacillus alcalophilusLW8 and its biotechnological applications.Journal of Taibah University for Science,8(4), 307-314.

Sardar, A. G., & Pathak, A. P. (2014). Exploring the microbiota of solar saltern of Mulund, Mumbai, India.Indian J. Mar. Sci.,43, 634-641.

Sharma, A., Satyanarayana, T. (2013). Microbial acid-stable amylases: Characteristics, genetic engineering and applications. Process Biochemistry, 48, 201–211.

Sharma, A., Satyanarayana, T. (2012). Production of Acid-Stable and High-Maltose-Forming α-Amylase of Bacillus acidicolaby Solid-State Fermentation and Immobilized Cells and Its Applicability in Baking. Appl Biochem Biotechnol, 168,1025–1034.

Sharma, A., Pandey, A., Shouche, Y. S., Kumar, B., & Kulkarni, G. (2009). Characterization and identification of Geobacillusspp. isolated from Soldhar hot spring site of Garhwal Himalaya, India.Journal of basic microbiology, 49(2), 187-194.

Sharma, B., Agrawal, R., Singhania, R. R., Satlewal, A., Mathur, A., Tuli, D., & Adsul, M. (2015). Untreated wheat straw: Potential source for diverse cellulolytic enzyme secretion by Penicillium janthinellumEMS-UV-8 mutant. Bioresource technology,196, 518-524.

Sharma, N. K. (2013). Chemical composition of oilseed cakes and deoiled cakes in Nepal.Online Journal of Animal and Feed Research (OJAFR),3(1), 74-76.

Sonalkar, V. V., Mawlankar, R., Ramana, V. V., Joseph, N., Shouche, Y. S., & Dastager, S. G. (2015). Bacillus filamentosussp. nov., isolated from sediment sample.Antonie van Leeuwenhoek,107(2), 433-441.

Onions, A.H.S., Allsopp, D. and Eggins, H.O.W. 1981. Smith’s Introduction to Industrial Mycology (Seventh Edition), Edward Arnold Publishing Co.

Visagie, C. M., Houbraken, J., Frisvad, J. C., Hong, S. B., Klaassen, C. H. W., Perrone, G., Seifert, K.A., Varga, J., Yaguchi, T. & Samson, R. A. (2014). Identification and nomenclature of the genus Penicillium.Studies in mycology,78, 343-371.

Downloads

Published

2016-04-30

How to Cite

Pathak, A. P., Devkatte, S. B., & Rathod, M. G. (2016). Production of Amylase by Penicillium sp. Using Solid State Fermentation Method and Inexpensive Agricultural Residues. International Journal For Research In Biology & Pharmacy, 2(4), 32–42. https://doi.org/10.53555/bp.v2i4.135