Ammonia Inhibition Level of Anaerobic Microbes Acclimated in the Anaerobic Digester Treating Pig Slurry under the High Ammonia Nitrogen

  • Young-Man Yoon Hankyong national university
  • Jae Ho AN Hankyong National University,
Keywords: Biogas, Anaerobic digestion, Ammonia inhibition, Methanogen

Abstract

A biochemical methane potential (BMP) assay was conducted to determine ammonia inhibition level of acclimated methanogens inoculated from an anaerobic digester in high total ammonia nitrogen (TAN) concentration. Anaerobic methane production was assessed by batch anaerobic reactor in mesophilic condition (38℃). Ammonium chloride (NH4C1) was added to adjust the TAN concentration to 1,000, 2,000, 3,000, 4,000, 5,000, 6,000, 7,000, 8,000, 9,000, 10,000 and 13,000 mg/L. The reactors of triplicate for each treatment and blank were incubated up to 50 days in a convection incubator. The biogas productions from the reactors below 7,000 mg TAN/L level were not different significantly, and the significant reduction of biogas production arose above from 8,000 mg TAN/L level. The theoretical methane potential of cellulose material, calculated by Buswell’s equation, was 0.414 Nm3/kg-VSadded. Ultimate methane potentials were in the range of 0.32-0.36 Nm3/kg-VSadded at the NH4+-N levels between 1,000 and 6,000 mg/kg. The ultimate methane potentials at the ammonia levels between 10,000 and 13,000 mg TAN/L showed the very low values of 0.10, 0.006 Nm3/kg-VSadded. Results of this study have presented that the methanogens acclimated at the high strength ammonium-nitrogen of about 4,000 – 5,000 mg/L could be tolerant to the ammonium nitrogen concentration of 7,000 mg/L. These results imply that the acclimation technology of methanogens is very useful for the improvement of digester microbial stability in the inhibitory anaerobic condition.

Downloads

Download data is not yet available.

Author Biographies

Young-Man Yoon, Hankyong national university

Department of Plant Life and Environmental Science, Hankyong National University,
The Republic of Korea

Jae Ho AN, Hankyong National University,

Department of Civil, Safety and Environmental Engineering, Hankyong National
University, The Republic of Korea

References

Albertson, O.E. (1961). Ammonia nitrogen and the anaerobic environment. J. Water Pollut. Control Fed. 33, 978-995. APHA (1998). Standard methods for the examination of water and wastewater. 20th, ed. American Public Health Association, Washington, DC, USA. Beuvink, J.M., Spoelstra, S.F., Hogendrop, R.J. (1992). An automated method of measuring the time course of gas production of feedstuffs incubated with buffered rumen fluid. Neth. J. Agri. Sci., 40, 401-407. Boe, K., Angelidaki, I. (2009). Serial CSTR digester configuration for improving biogas

production from manure. Wat. Res. 43, 166-172. Boyle, W.C. (1976). Energy recovery fromsanitary landfills―a review. In: Schlegel, H.G., and, J., Barnea (Eds.), Microbial Energy Conversion. Pergamon Press Oxford,

pp,119–138. Costa, J.C., Barbosa, S.G., Alves, M.M., Sousa, D.Z. (2012). Themochemical pre- and biological co-treatments to improve hydrolysis and methane production from poultry litter. Bioresour. Technol. 111, 141-147.

Krylova, N.I., Khabibouline, R.E., Naumova, R.P., Nagel, M.A. (1997). The influenced of ammonium and methods for removal during the anaerobic treatment of poultry manure. J. Chem. Techno. Biotechnol., 70, 99-105.

Labatut, R.A., Angenent, L.T., Scott N.R. (2011). Biochemical methane potential and biodegradability of complex organic substrates. Bioresour. Technol., 102, 2255-2264. Koster, I.W. (1986). Characteristics of the pH-influenced adaptation of methanogenic sludge to ammonium toxicity. J. Chem. Technol. Biotechnol., 36, 445-455. Krylova, N.I., Khabiboulline, R.E., Naumova, R.P., Nagel, M.A. (1997). The influence of ammonium and methods for removal during the anaerobic treatment of poultry manure. J. Chem. Tech. Biotechnol., 70, 99-105. Labatut, R.A., Angenent, L.T., Scott, N.R. (2011). Biochemical methane potential and biodegradability of complex organic substrates. Bioresour. Technol., 102, 2255-2264. McCarty, P.L. (1964a). Anaerobic waste treatment fundamentals - Part Three: Toxic materials and their control. Public Works, 95, 91-94. McCarty, P.L. (1964b). Anaerobic waste treatment fundamentals - Part Four: Process design. Public Works, 95, 95-99. Melbinger, N.R., Donnellon, J. (1971). Toxic effects of ammonia nitrogen in high-rate digestion. J. Water Pollut Control Fed., 43, 1658-1670.

Shelton, D.R., Tiedije, J.M. (1984). General method for determining anaerobic biodegradation potential., 47, 850-857. Symons, G.E., Buswell, A.M. (1933). The methane fermetnation of carbohydrates. Journal of Am. Chem.Soc., 55, 2028-2036. van Velsen, A.F.M. (1979). Adaptation of methanogenic sludge to high ammonianitrogen concentrations. Water Res., 13, 995-999. Williams, A., Amat-Marco, M., Collins, M.D. (1996). Pylogenetic analysis of

Butyrivibrio strains reveals three distinct groups of species within the Clostridium subphylm of Gram-positive bacteria. Int. J. Syst. Bacterol., 46, 195-199. Yenigün, O., Demirel, B. (2013). Ammnia inhibition in anaerobic digestion: A review. Process Biochem., 48, 901-911. Yoon, Y.M., Kim, Y.J., Kim, C.H. (2009). The evaluation of economical efficiency to composting and liquefying process of biomass discharged in pig breeding. Agr. Econ.

, 39-62. Yu, Y., Lee, C., Hwang, S. (2005). Analysis of community structures in anaerobic processes using a quantitative real-time PCR method. Wat. Sci. Technol. 52, 85-91.

Published
2019-03-04
How to Cite
Yoon, Y.-M., & AN, J. H. (2019). Ammonia Inhibition Level of Anaerobic Microbes Acclimated in the Anaerobic Digester Treating Pig Slurry under the High Ammonia Nitrogen. International Journal For Research In Agricultural And Food Science (ISSN: 2208-2719), 5(2), 40-59. Retrieved from https://gnpublication.org/index.php/afs/article/view/832