Potential Hazard Levels of Lead and Cadmium in Muscles of the River Nile Catfish (Clarias gariepinus) Available in Markets in Assiut City, Egypt
Keywords:
Heavy metals, lead, cadmium, catfish, Clarias gariepinusAbstract
The objective of current study was to determine the levels of lead (Pb) and cadmium (Cd) in the muscular tissue of the River Nile catfish "Clarias gariepinus", collected randomly from fishsellers in Assiut city, Egypt. A total of 50 samples were purchased, inspected and prepared for metal analysis. Inductively Coupled Plasma Emission Spectrometer was used for quantitative determination of lead and cadmium in the samples. The obtained concentrations for Pb ranged from 0.02 to 4.0 with a mean value of 1.29±0.17 mg Kg-1 , while that of cadmium ranged from 0.03 to 1.05 with a mean value of 0.19±0.04 mg Kg-1 . Most of the samples "66% and 76%" had residues of lead and cadmium exceeded the permissible limit (0.3 and 0.03 mg Kg-1 ) set for fish by Egyptian
Organization for Standardization and Quality, respectively. In conclusion, the flesh of Clarias gariepinus fish sold in markets of Assiut city, constitute a potential public health hazard considering lead toxicity and to a less degree for cadmium
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Abd El-Azeem, M.H.; Hassan, A.M.; Hussein, M.M., Abd El-Wahed, Z.H. and Saleh, R.E. (2012): Some heavy metal pollutants in fish farms and effect of garlic on their bioaccumulation in Oreochromis niloticus. Suez Canal Vet. Med. J., 17(1): 57-69. Abd El-Malek, A.M. and Ammar, M.A. (2013): Lead and cadmium in Tilapia fish Oreochromis
niloticus (Bolty) tissues. Zag. Vet. J. 41(3):50-56. Aderinola, O.J.; Kusemiju, V. and Clarke, E.O. (2012): Trace metal distribution in surface water, sediment, and tissues of freshwater catfish (Clarias gariepinus), from Oke-Afa Canal, Lagos Nigeria. Inter. J. Geograph. Geo., 1(1): 10-22. Akwiri, O.C.; Raburu1, P.O.; Okeyo, O.J.B.; F. Ramesh, F. and Onyangore, F.O. (2016): Concentration of selected heavy metals in sediments and liver of wild African Catfish (Clarias gariepinus) in Lake Kanyaboli, Kenya. Ann. Res. Rev. Biol., 11(2):1-8 ATSDR (Toxicological Profile for Lead) (1999). US Department of Health and Human Services, Public Health Services 205-93-0606 Authman, M.M.N.; Abbas, W.A. and Gaafar, A.Y. (2012): Metals concentrations in Nile tilapia Oreochrmis niloticus (Linnaeus, 1758) from illegal fish farm in Al-Minufiya Province, Egypt, and their effects on some tissues structures. Ecotoxicol. Environ. Safe., 84:163-172. Başyiğit, B. and Tekin-Özan, S. (2013): Concentrations of some heavy metals in water, sediment,and tissues of pikeperch (Sander lucioperca) from Karataş Lake related to physico-chemical
parameters, fish size, and seasons. Pol. J. Environ. Stud., 22(3): 633-644. Bayomy, Hala, M.; Rozan, M.A. and Ziena, H.M. (2015): Lead and cadmium contents in Nile water, tilapia and catfish from Rosetta branch, River Nile, Egypt. J. Food Dairy Sci., Mansoura Univ. 6(4):253-262. Ben-Salem, Zohra and Ayadi, H. (2016): Assessment of heavy metal contamination levels and toxicity in sediments and fishes from the Mediterranean Sea (southern coast of Sfax, Tunisia). Environ. Sci. Pollut. Res., DOI 10.1007/s11356-016-6534-3. Bob-Manuel, F.G.; Wokoma, O.A.F. and Friday, U. (2015): Heavy metal conc entration in some organs of Clarias gariepinus (African Catfish) from Okilo Creek, Rivers State, Nigeria. Annals of Biol. Res., 6 (11): 68-71. Castro-Gonzeza, M.I. and Mendez-Armentab, M. (2008): Heavy metals: implications associated to fish consumption. Environ. Toxicol. Pharmacol. 26, 263-271.
Chakraborty, R.; Dey, S.; Dkhar, P.S.; Ghosh, D.; Singh, S.; Sharma, D.K. and Myrboh, B. (2003): Accumulation of heavy metals in some freshwater fishes from eastern India and its possible impact on human health. Poll Res., 22(3): 353-358. Chandrasekhar, K.; Chary, N.S.; Kamala, C.T.; Suman Raj, D.S. and Sreenivasa Rao, A. (2003):
Fractionation studies and bioaccumulation of sediment bound heavy metals in Kolleru Lake by edible fish. Environ Int., 29: 1001-1008.Codex Alimentarius Commission Procedural Manual. (2001): Maximum residual limits (MRLs) for pesticides. CAC/MRL, Rev. 1-2001. Cunningham, W.P. and Saigo, W.B. (1997): Environmental science: A global concern. 4th ed. WCB New York USA. Darwish, A.M.; El-Mossalami, M.K. and ElBassuony, R.A. (2003): Quality assurance of some fatty fishes. Assuit Vet. M. J., 49(98): 79-96. Dimari, G. A.; Abdulkarim, F. I.; Akan, J. C. and Garba,S.T. (2008): Metal concentrations in tissues of Tilapia galier, Clarias lazera, and Osteoglosidae caught from Alau Dam, Maiduguri, Borno State, Nigeria. Amer. J. Environ Sci., 4 (4): 473-479. Doherty, V.F.; Ogunkuade, O.O. and Kanife, U.C. (2010): Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in some selected fishes in Lagos, Nigeria. American-Eurasian J. Agric. Environ. Sci., 7 (3): 359-365. EC "European Community". (2006): Commission Regulation 2006/1881/EC of 19 December 2006
replacing regulation (EC) 466/2001 setting maximum levels for certain contaminants in foodstuffs. OJ L 364/5-24, Brussels, 20.12.2006. Edward, J. B.; Idowu, E. O.; Oso, J. A. and Ibidapo, O. R. (2013): Determination of heavy metalconcentration in fish samples, sediment and water from Odo-Ayo River in Ado-Ekiti, EkitiState, Nigeria. Int. J. Environ. Monit. Anal., 1(1): 27-33. Elijah, L.K.; Young, E. and Tobin, E.A. (2016): Bioavailability of heavy metals in epipelagic sediments and tissues of African Catfish (Clarias gariepinus) of the Kolo Creek, Bayelsa State,
Nigeria. J. Multi. Eng. Sci. Tech., 3(1): 3803-3807. Elnabris, K.J.; Muzyed, S.K. and El-Ashgar, N.M. (2013): Heavy meal concentrations in some commercially important fishes and their contribution to heavy metals exposure in Palestinian people of Gaza Strip (Palestine). J. Ass. Arab. Univ. Basic Appl. Sci., 13:44-51. El-Shafei, H.M. (2015): Some heavy metals concentration in water, muscles and gills of Tilapia niloticus as biological indicator of Manzala Lake pollution. J. Aquac. Res. Develop. 6(9):1-5. Emara, M.M.; Farag, R.S.; Dawah, A.A. and Fathi, M. (2015): Assessment of heavy metals concentration in water and edible Nile Tilapia (Oreochromis niloticus) from two fish farms irrigated with different water sources, Egypt. Int. J. Environ. 4(1):108-115. Eneji, I.S.; Ato, R.S. and Annune, P.A. (2011): Bioaccumulation of heavy metals in fish (Tilapia zilli and Clarias gariepinus) organs from River Benue, North Central Nigeria. Pak. J. Anal. Environ. Chem., 12 (1 & 2): 25-31. EOS "Egyptian Organization for Standardization and Quality". (2010): Maximum levels for certain contaminants in foodstuffs. ES7136/2010.
FAO (1995): Quality and quality changes in fresh fish. Huss, H. H. (edit), FAO Fisheries Technical Paper No. 348.
FAO (2010): The international fish trade and world fisheries. http://www.fao.org/fileadmin/user_upload/newsroom/docs/fact_sheet_fish_trade_en.pd FAO (2011): Fisheries and aquaculture markets in the Middle East. Towers, L. (edit), FAO Fisheries Technical Paper. http://www.fao.org/in-action/globefish/fishery-information/resource-detail/en/c/338542/ FAO/WHO (1989): Evaluation of certain additives and the contaminants mercury, lead and cadmium. WHO Technical Report, Series No. 505. FAO/WHO (2004): Summary of evaluation performed by the Joint FAO/WHO Expert Committee
on Food Additives (JECFA 1956-2003). Finerty, M.W.; Madden, J.D.; Feagley, S.E. and Grodner, R.M. (1990): Effect of environs and seasonality on metal residues in tissues of wild and pond-raised crayfish in Southern Louisiana.
Arch. Environ. Contam. Toxicol., 19:94-100. Gavaghan, H. (2002): Lead unsafe at any level. Bull. World Heal. Org., 80(1): 82-202. Goos, H.J. and Richter, C.J. (1996): Internal and external factors controlling reproduction in
African Catfish (Clarias gariepinus) In: The biology and culture of Catfish aquatic living resources, Legendre, M. and Proteau, J.P. (Eds.)" Vol. 9. Academic Publisher, UK, pp: 46-58Gupta, A.; Rai, D.K.; Pandey, R.S. and Sharma, B. (2009): Analysis of some heavy metals in the riverine water, sediments and fish from River Ganges at Allahabad. Environ. Monit. Assess., 157: 449-458. Habib, S.A. and Samah, A.M.A.S. (2013): Effect of heavy metals pollution on protein biosynthesis in Catfish. J. Water Res. Prot., 5: 555-562. Hamed, Y.A.; Abdelmonein, T.S.; Elkiki, M.H.; Hassan, M.A. and Berndtsson, R. (2013): Assessment of heavy metals pollution and microbial contamination in water, sediments and fish of Lake Manzala, Egypt. Life Sci. J., 10(1):86-99. Ibrahem, M.D. (2012): Experimental exposure of African catfish Clarias gariepinus (Burchell, 1822) to phenol: Clinical evaluation, tissue alterations and residue assessment. J. Adv. Res., 3: 177-183. Ibrahim , A.Th. (2007): Patterns of heavy metals distribution and pollution-induced changes in some organs of three Nile fish species from Assiut, Egypt. M.Sc. Thesis, Fac. of Sci. Assiut Univ., Egypt. Ibrahim, A.T.A. and Omar, H.M. (2013): Seasonal variation of heavy metals accumulation in
muscles of the African Catfish Clarias gariepinus and in River Nile water and sediments at
Assiut Governorate, Egypt. J. Biol. Earth Sci., 3(2): B236-B248.
Ibrahim, A.T.A.; Wassif, E.T. and Alfons, M.S. (2016): Heavy metals assessment in water,
sediments and some organs of Oreochromis niloticus under the impact of sewage water. J.
Heavy Met. Toxicity Dis. 1(4):1-7.
Ibrahim, Seham, A.; Authman, M.M.N.; Gaber, Hanan, S. and El-Kasheif, M.A. (2013):
Bioaccumulation of heavy metals and their histopathological impact on muscles of Clarias
gariepinus from El-Rahawy drain, Egypt. Int. J. Environ. Sci. Eng., 4: 57-73.
Ijeoma, K.H.; Onyoche, N.E.; Uju, O.V. and Chukwuene, I.F. (2015): Determination of heavy
metals in fish (Clarias gariepinus) organs from Asaba major markets, Delta State, Nigeria.
Amer. Chem. Sci. J., 5(2): 135-147.
Jarup, L. (2003): Hazards of heavy metal contamination. British Med. Bull., 68:167- 182.
Jinadasa, B.K.K.K., and Edirisinghe, E.M.R.K.B. (2013): Cadmium, lead and total mercury in
Tilapis sp. In Sri Lankan reservoirs. Food Addit. Contam. Part B. 7(2):90-94.
Juniato, Zahidah and Apriliani, I.M. (2017): Evaluation of heavy metal contamination in various
fish meat from Cirata Dam, West Java, Indonesia. AACL Bioflux. 10(2):241-246.
Kaoud, H.A. and El-Dahshan, A.R. (2010): Bioaccumulation and histopathological alterations of
heavy metals in Oreochromis niloticus fish. Nat. and Sci., 8(4):147-156.
Karadede-Akin, H. and Unlu, E. (2007): Heavy metal concentrations in water, sediment, fish and
some benthic organisms from Tigris River, Turkey. Environ. Monit. Assess., 131:323-337.
Malhat, F. (2011): Distribution of heavy metal residues in fish from the River Nile tributaries in
Egypt. Bull. Environ. Contam. Toxicol., 87:163–165.
Mansour, S.A. and Sidky, M.M. (2002): Ecotoxicological studies. 3. Heavy metals contaminating
water and fish from Fayoum Governorate, Egypt. Food Chem. 78:15-22.
Markert, B.; Pedrozo, F.; Geller, W.; Friese, K.; Korhammer, S.; Baffico, G.; Diaz, M.; and Wolfl,
S. (1997): A contribution to the study of the heavy-metal and nutritional element status of some
lakes in the Southern Andes of Patagonia (Argentina). Sci. Total Environ., 206(1): 1-5.
Nhiwatiwa, T.; Barson, M.; Harrison, A.P.; Utete, B. and Copper, R.G. (2017): Metal
concentrations in water, sediment and sharp tooth catfish Clarias gariepinus from three periurban rivers in the Upper Manyame catchment, Zimbabwe. Afr. J. Aqua. Sci., 36 (3): 243-252.
Nzeve, J.K.; Njuguna, S.G. and Kitur, E.C. (2014): Bioaccumulation of heavy metals in Clarias
gariepinus and Oreochromis spirulus niger from Masinga Reservoir, Kenya. J. Environ. Sci.
Toxico. Food Tech., 8 (10): 58-63.
Ololade, I.A.; Lajide, L.; and Amoo, I.A. (2007): Enrichment of heavy metals in sediments as
pollution indicator of the aquatic ecosystem. Pak. J. Sci. Ind. Res., 50(1): 27-35.
Olusola, A.V.; Folashade, P.A. and Ayoade, O.I. (2012): Heavy metal (lead, cadmium) and
antibiotic (tetracycline and chloramphenicol) residues in fresh and frozen fish types (Clarias .gariepinus, Oreochromis niloticus) in Ibadan, Oyo State, Nigeria. Pak. J. Biol. Sci. 15(18): 895-
Omozokpia, J.A.; Ajai, A.I.; Ndamitso, M.M.; Jacob, J.O.; Akoma, A. and Tanko, E. (2015): Heavy
metals accumulation in water, sediments and Catfish (Clarias gariepinus) from two fishing
settlements along River Kaduna in Niger State, Nigeria. Br. J. Appl. Sci. Tech., 11(1): 1-13.
Orosun, M.M.; Tchokossa, P.; Orosun, R.O.; Akinyose, F.C.; Ige, S.O. and Oduh, V.O. (2016):
Determination of selected heavy metals and human health risk assessment in fishes from Kiri
Dam and River Gongola, Northeastern Nigeria. J. Phys. Chem. Biophys., 6 (5): 1-6.
Osman, A. and Kloas, W. (2010): Water quality and heavy metal monitoring in water, sediments,
and tissues of the African Catfish Clarias gariepinus (Burchell, 1822) from the River Nile,
Egypt. J. Environ. Prot., 1: 389-400.
Patricia, U.A. and Boma, J. (2013): Cadmium (Cd) and lead (Pb) in Penaeus notialis purchased
from Creek Road market, Port Harcourt, Nigeria: Risk assessment of Cd from consumption of
P. notialis. Int. J. Fish. and Aquat. Sci., 2(2): 38-42.
Raeisi, S.; Rad, J.S.; Rad, M.S. and Zakariaei, H. (2014): Analysis of heavy metals content in water,
sediments and fish from the Gorgan bay, southeastern Caspian sea, Iran. Int. J. Adv. Biol.
Biomed. Res., 2(6): 2162-2172.
SASO "Saudi Arabia Standards Organization" (1997): Maximum limits of contaminating metallic
elements in foods. Riyadh, Saudi Arabia.
Shaltout, F.A.; Mohammed, H.F. and Saber, E. (2015): Detection of some heavy metals in fish
(Tilapia nilotica and Clarias lazera) at Menofia Governorate. Benha Vet. M. J., 29(1):124-128.
Sim, S.F.; Ling, T.Y.; Nyanti, L.; Gerunsin, N.; Wong, Y.E. and Kho, L.P. (2016): Assessment of
heavy metals in water, sediment, and fishes of a Large Tropical Hydroelectric Dam in Sarawak,
Malaysia. J. Chem., 1-10. http://dx.doi.org/10.1155/2016/8923183.
Suhaimi, F.; Wong, S.P.; Lee, V.L.L. and Low, L.K. (2006): Heavy metals in fish and shellfish
found in local wet markets. Singapore J. Prim. Ind., 32: 1-18.
Tabinda, A.B.; Bashir, S.; Yasar, A. and Hussain, M. (2013): Metals concentrations in the riverine
water, sediments and fishes from River Ravi at Balloki Headworks. J. Anim. Plant Sci., 23(1):
-84.
Teugels G. A. (1986): Systemic revision of the African species of the genus Clarias (Pisces:
Clariidae). Ann Musee Royal l’Afrique Centr., 247: 1-199.
Tiimub, B.M. and Afua, M.A.D. (2013): Determination of selected heavy metals and iron
concentration in two common fish species in Densu River at Weija district in Grater Accra
region of Ghana. Amer. Int. J. Biol., 1(1): 45-55.
Titilayo, A.O. and Olufemi, A.O. (2014): Bioaccumulation of heavy metals in fish (Clarias
gariepinus) organs from selected streams in South Western Nigeria. 2nd International
Conference on Sustainable Environment and Agriculture, Singapore, IPCBEE, Vol. 76 (10): 47-
Turkmen, M.; Turken, A.; Tepe, Y.; Tore, Y. and Ates, A. (2009): Determination of metals in fish
species from Aegean and Mediterranean Seas. Food Chem., 113: 233-237.
Udiba, U.U.; Balli, G.; Odey, M.O.; Umar, S.M.; Ashade, N.O.; Odeke, E.H.; Bala, M. and Dawaki,
S.I. (2014): Contaminants levels of African Cat fish (Clarias gariepinus) tissues: A comparative
study of River Galma, River Kubanni and fish farms in Zaria, Nigeria. Merit. Res. J. Environ.
Sci. Toxico., 2(8): 156-166.
Vieira, C.; Morais, S.; Ramos, S.; Delerue-Matos, C. and Oliveira, M.B.P.P. (2011): Mercury,
cadmium, lead and arsenic levels in three pelagic fish species from the Atlantic Ocean: Intraand inter-specific variability and human health risks for consumption. Food Chem. Toxicol., 49:
-932.
WHO (1985): Global environmental monitoring system. Guidelines for the study of dietary intakes
of chemical contaminants. Geneva, WHO, Offset publication No 87.
WHO (2008): Guidelines for drinking water quality. Third Edition Incorporating 1st and 2nd
Addenda Recommendation WHO. Geneva, 1:130. 25
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